Terminator

Part:BBa_K112710:Design

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-10-21)


bca1041


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Construction of <Bca1041> basic part K112710

PCR of dv023/dv024  pSB1AG0-Bca1041                   (122bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+122, L)
Product is pBca1256-K112710
-----------------------------------------------
dv023 Forward biobricking of Bca1041
cgataGAATTCatgAGATCTgCcggcttatcGgtcagtttc

dv024 Reverse biobricking of Bca1041
cgttaGGATCCatttcttttgggtatagcgtcgtgg

References